2.43
1,65
1,98
0,73
1.88
1.81
2.43
2.2 Standartvielas, ko izmanto relatīvās molekulmasas sadalījuma kalibrēšanas līknē: insulīns, mikopeptīdi, glicīns-glicīns-tirozīns-arginīns, glicīns-glicīns-glicīns
3 Instrumenti un aprīkojums
23.2
21.4
22.2
16.1
22.3
20.8
23.9
27,5
Kopumā aminoskābju īpatsvars Sustar produktos ir lielāks nekā Zinpro produktos.
8. daļa Lietošanas sekas
Dažādu mikroelementu avotu ietekme uz dējējvistu ražošanas rādītājiem un olu kvalitāti dēšanas perioda beigās
Ražošanas process
Mērķtiecīga helātu tehnoloģija
Bīdes emulgācijas tehnoloģija
Spiediena izsmidzināšanas un žāvēšanas tehnoloģija
Saldēšanas un mitruma atdalīšanas tehnoloģija
Uzlabota vides kontroles tehnoloģija
A pielikums: Peptīdu relatīvās molekulmasas sadalījuma noteikšanas metodes
Standarta pieņemšana: GB/T 22492-2008
1. testa princips:
Tas tika noteikts ar augstas veiktspējas gēla filtrācijas hromatogrāfiju. Tas ir, izmantojot porainu pildvielu kā stacionāro fāzi, pamatojoties uz atdalāmo parauga komponentu relatīvās molekulmasas lieluma atšķirību, kas noteikta pie peptīdu saites ultravioletā absorbcijas viļņa garumā 220 nm, izmantojot speciālu datu apstrādes programmatūru relatīvās molekulmasas sadalījuma noteikšanai ar gēla filtrācijas hromatogrāfiju (t. i., GPC programmatūru), hromatogrammas un to dati tika apstrādāti, aprēķināti, lai iegūtu sojas pupiņu peptīda relatīvās molekulmasas lielumu un sadalījuma diapazonu.
2. Reaģenti
Eksperimentālajam ūdenim jāatbilst sekundārā ūdens specifikācijai GB/T6682 standartā, un reaģentiem, izņemot īpašus noteikumus, jābūt analītiski tīriem.
2.1 Reaģenti ietver acetonitrilu (hromatogrāfiski tīru), trifluoretiķskābi (hromatogrāfiski tīru),
2.2 Standartvielas, ko izmanto relatīvās molekulmasas sadalījuma kalibrēšanas līknē: insulīns, mikopeptīdi, glicīns-glicīns-tirozīns-arginīns, glicīns-glicīns-glicīns
3 Instrumenti un aprīkojums
3.1 Augstas veiktspējas šķidruma hromatogrāfs (HPLC): hromatogrāfiska darbstacija vai integrators ar UV detektoru un GPC datu apstrādes programmatūru.
3.2 Mobilās fāzes vakuuma filtrācijas un degazācijas iekārta.
3.3 Elektroniskie svari: graduēta vērtība 0,000 1 g.
4 darbības soļi
4.1 Hromatogrāfiskie apstākļi un sistēmas adaptācijas eksperimenti (atsauces apstākļi)
- 4.1.1 Hromatogrāfiskā kolonna: TSKgelG2000swxl300 mm × 7,8 mm (iekšējais diametrs) vai citas tāda paša tipa gēla kolonnas ar līdzīgu veiktspēju, kas piemērotas olbaltumvielu un peptīdu noteikšanai.
- 4.1.2 Mobilā fāze: acetonitrils + ūdens + trifluoretiķskābe = 20 + 80 + 0,1.
- 4.1.3 Noteikšanas viļņa garums: 220 nm.
- 4.1.4 Plūsmas ātrums: 0,5 ml/min.
- 4.1.5 Noteikšanas laiks: 30 minūtes.
- 4.1.6 Parauga injekcijas tilpums: 20 μL.
- 4.1.7 Kolonnas temperatūra: istabas temperatūra.
- 4.1.8 Lai hromatogrāfijas sistēma atbilstu noteikšanas prasībām, tika noteikts, ka iepriekš minētajos hromatogrāfijas apstākļos gēla hromatogrāfijas kolonnas efektivitāte, t. i., teorētiskais plākšņu skaits (N), nav mazāka par 10 000, kas aprēķināta, pamatojoties uz tripeptīdu standarta (glicīns-glicīns-glicīns) maksimumiem.
- 4.2 Relatīvās molekulmasas standarta līkņu veidošana
- Iepriekš minētie dažādu relatīvo molekulmasu peptīdu standartšķīdumi ar masas koncentrāciju 1 mg/ml tika sagatavoti, izmantojot mobilās fāzes saskaņošanu, sajaukti noteiktā proporcijā un pēc tam filtrēti caur organiskās fāzes membrānu ar poru izmēru 0,2 μm~0,5 μm, ievadīti paraugā un pēc tam iegūtas standartu hromatogrammas. Relatīvās molekulmasas kalibrēšanas līknes un to vienādojumi tika iegūti, uzzīmējot relatīvās molekulmasas logaritmu pret aiztures laiku vai izmantojot lineāro regresiju.
4.3 Parauga apstrāde
Precīzi nosver 10 mg parauga 10 ml mērkolbā, pievieno nedaudz kustīgās fāzes, ultraskaņas krata 10 minūtes, lai paraugs būtu pilnībā izšķīdis un sajaukts, atšķaida ar kustīgo fāzi līdz atzīmei un pēc tam filtrē caur organiskās fāzes membrānu ar poru izmēru 0,2 μm~0,5 μm, un filtrātu analizē saskaņā ar hromatogrāfijas nosacījumiem, kas aprakstīti A.4.1.
- 5. Relatīvās molekulmasas sadalījuma aprēķins
- Pēc 4.3. punktā sagatavotā parauga šķīduma analīzes 4.1. punktā noteiktajos hromatogrāfijas apstākļos parauga relatīvo molekulmasu un tās sadalījuma diapazonu var iegūt, aizstājot parauga hromatogrāfijas datus ar GPC datu apstrādes programmatūru kalibrēšanas līknē 4.2. Dažādu peptīdu relatīvo molekulmasu sadalījumu var aprēķināt, izmantojot pīķa laukuma normalizācijas metodi pēc formulas: X=A/A kopā×100
- Formulā: X - relatīvās molekulmasas peptīda masas daļa kopējā peptīdā paraugā, %;
- A - relatīvās molekulmasas peptīda pīķa laukums;
- Kopējais A — katra relatīvās molekulmasas peptīda pīķa laukumu summa, kas aprēķināta ar precizitāti līdz vienai zīmei aiz komata.
- 6 Atkārtojamība
- Absolūtā starpība starp divām neatkarīgām noteikšanām, kas iegūtas atkārtojamības apstākļos, nedrīkst pārsniegt 15 % no abu noteikšanu vidējā aritmētiskā.
- B pielikums: Brīvo aminoskābju noteikšanas metodes
- Standarta pieņemšana: Q/320205 KAVN05-2016
- 1.2 Reaģenti un materiāli
- Ledus etiķskābe: analītiski tīra
- Perhlorskābe: 0,0500 mol/l
- Indikators: 0,1% kristālvioletais indikators (ledus etiķskābe)
- 2. Brīvo aminoskābju noteikšana
Paraugi tika žāvēti 80 °C temperatūrā 1 stundu.
Ievietojiet paraugu sausā traukā, lai tas dabiski atdzistu līdz istabas temperatūrai vai atdzesētos līdz lietošanas temperatūrai.250 ml sausā koniskā kolbā iesver aptuveni 0,1 g parauga (ar precizitāti līdz 0,001 g).Ātri pārejiet pie nākamās darbības, lai paraugs neuzsūktu apkārtējā mitruma.Pievienojiet 25 ml ledus etiķskābes un labi samaisiet ne ilgāk kā 5 minūtes.Pievienojiet 2 pilienus kristālvioletā indikatoraTitrē ar 0,0500 mol/l (±0,001) perhlorskābes standarta titrēšanas šķīdumu, līdz šķīduma krāsa mainās no violetas līdz gala punktam.
Pierakstiet patērētā standarta šķīduma tilpumu.
- Vienlaikus veiciet tukšo mēģinājumu.
- 3. Aprēķins un rezultāti
- Brīvo aminoskābju saturu X reaģentā izsaka kā masas daļu (%) un aprēķina pēc formulas: X = C × (V1-V0) × 0,1445/M × 100%, izmantojot šādu formulu:
- C - Standarta perhlorskābes šķīduma koncentrācija molos uz litru (mol/L)
- V1 — tilpums, kas izmantots paraugu titrēšanai ar standarta perhlorskābes šķīdumu, mililitros (ml).
- Vo - tilpums, kas izmantots titrēšanas tukšajā paraugā ar standarta perhlorskābes šķīdumu, mililitros (ml);
M — parauga masa gramos (g).
| 0,1445: vidējā aminoskābju masa, kas atbilst 1,00 ml standarta perhlorskābes šķīduma [c (HClO4) = 1,000 mol / L]. | 4.2.3 Cērija sulfāta standarta titrēšanas šķīdums: koncentrācija c [Ce(SO4)2] = 0,1 mol/l, sagatavots saskaņā ar GB/T601. | |
| Standartu pieņemšana: Q/70920556 71-2024 | 1. Noteikšanas princips (Fe kā piemērs) | Aminoskābju dzelzs kompleksiem ir ļoti zema šķīdība bezūdens etanolā, un brīvo metālu joni šķīst bezūdens etanolā, aminoskābju dzelzs kompleksu helātu veidošanās ātruma noteikšanai tika izmantota abu šķīdības atšķirība bezūdens etanolā. |
| Formulā: V1 - cērija sulfāta standartšķīduma tilpums, kas patērēts testa šķīduma titrēšanai, ml; | Bezūdens etanols; pārējais atbilst GB/T 27983-2011 4.5.2. punktam. | 3. Analīzes posmi |
| Veiciet divus paralēlus mēģinājumus. Nosveriet 0,1 g parauga, kas žāvēts 103±2 °C temperatūrā 1 stundu ar precizitāti līdz 0,0001 g, pievienojiet 100 ml bezūdens etanola, lai izšķīdinātu, filtrējiet, filtrēto atlikumu vismaz trīs reizes mazgājiet ar 100 ml bezūdens etanola, pēc tam pārnesiet atlikumu 250 ml koniskā kolbā, pievienojiet 10 ml sērskābes šķīduma saskaņā ar GB/T27983-2011 4.5.3. punktu un pēc tam veiciet šādas darbības saskaņā ar GB/T27983-2011 4.5.3. punktu “Sildiet, lai izšķīdinātu, un pēc tam ļaujiet atdzist”. Vienlaikus veiciet tukšo mēģinājumu. | 4. Kopējā dzelzs satura noteikšana | 4.1 Noteikšanas princips ir tāds pats kā GB/T 21996-2008 4.4.1. punktā. |
4.2. Reaģenti un šķīdumi
| 4.2.1 Jaukta skābe: Pie 700 ml ūdens pievieno 150 ml sērskābes un 150 ml fosforskābes un labi samaisa. | 4.2.2 Nātrija difenilamīna sulfonāta indikatora šķīdums: 5 g/l, sagatavots saskaņā ar GB/T603. | 4.2.3 Cērija sulfāta standarta titrēšanas šķīdums: koncentrācija c [Ce(SO4)2] = 0,1 mol/l, sagatavots saskaņā ar GB/T601. | |
| 4.3 Analīzes posmi | Veiciet divus paralēlus mēģinājumus. Nosveriet 0,1 g parauga ar precizitāti līdz 0,20001 g, ievietojiet to 250 ml koniskā kolbā, pievienojiet 10 ml jauktas skābes, pēc izšķīdināšanas pievienojiet 30 ml ūdens un 4 pilienus nātrija dianilīna sulfonāta indikatora šķīduma un pēc tam veiciet šādas darbības saskaņā ar GB/T21996-2008 4.4.2. punktu. Vienlaikus veiciet tukšo mēģinājumu. | 4.4 Rezultātu attēlošana | Aminoskābju dzelzs kompleksu kopējais dzelzs saturs X1, izteikts procentos (%), tika aprēķināts pēc formulas (1): |
| X1 = (V - V0) × C × M × 10⁻³ × 100 | V0 - cērija sulfāta standartšķīdums, kas patērēts tukšā šķīduma titrēšanai, ml; | V0 - cērija sulfāta standartšķīdums, kas patērēts tukšā šķīduma titrēšanai, ml; | C - Cērija sulfāta standarta šķīduma faktiskā koncentrācija, mol/L5. Dzelzs satura aprēķināšana helātosDzelzs saturs X2 helātā, izteikts procentos (%), tika aprēķināts pēc formulas: x2 = ((V1-V2) × C × 0,05585)/m1 × 100 |
| Formulā: V1 - cērija sulfāta standartšķīduma tilpums, kas patērēts testa šķīduma titrēšanai, ml; | V2 — tukšā šķīduma titrēšanai patērētais cērija sulfāta standartšķīdums, ml;nom1 — parauga masa, g. Kā noteikšanas rezultātus ņem paralēlās noteikšanas rezultātu aritmētisko vidējo vērtību, un paralēlās noteikšanas rezultātu absolūtā starpība nepārsniedz 0,3 %. | 0,05585 — dzelzs(II) masa gramos, kas atbilst 1,00 ml cērija sulfāta standartšķīduma C[Ce(SO4)2.4H20] = 1,000 mol/l.nom1 — parauga masa, g. Kā noteikšanas rezultātus ņem paralēlās noteikšanas rezultātu aritmētisko vidējo vērtību, un paralēlās noteikšanas rezultātu absolūtā starpība nepārsniedz 0,3 %. | 6. Helātu veidošanās ātruma aprēķināšanaHelātu veidošanās ātrums X3, vērtība izteikta procentos, X3 = X2/X1 × 100C pielikums: Zinpro helātu veidošanās ātruma noteikšanas metodes |
Standarta pieņemšana: Q/320205 KAVNO7-2016
1. Reaģenti un materiāli
a) Ledus etiķskābe: analītiski tīra; b) Perhlorskābe: 0,0500 mol/l; c) Indikators: 0,1 % kristālvioletais indikators (ledus etiķskābe)
2. Brīvo aminoskābju noteikšana
2.1 Paraugi tika žāvēti 80°C temperatūrā 1 stundu.
2.2 Ievietojiet paraugu sausā traukā, lai tas dabiski atdzistu līdz istabas temperatūrai vai atdzesētos līdz lietošanas temperatūrai.
2.3. Aptuveni 0,1 g parauga (ar precizitāti līdz 0,001 g) iesver 250 ml sausā koniskā kolbā.
2.4 Ātri pārejiet pie nākamās darbības, lai paraugs neuzsūktu apkārtējā mitruma.
2.5 Pievienojiet 25 ml ledus etiķskābes un labi samaisiet ne ilgāk kā 5 minūtes.
2.6 Pievienojiet 2 pilienus kristālvioletā indikatora.
2.7 Titrē ar 0,0500 mol/L (±0,001) perhlorskābes standarta titrēšanas šķīdumu, līdz šķīduma krāsa mainās no violetas uz zaļu 15 sekundes, nemainot krāsu kā beigu punktu.
2.8 Pierakstiet patērētā standarta šķīduma tilpumu.
2.9 Vienlaikus veiciet tukšo mēģinājumu.
- 3. Aprēķins un rezultāti
- Katalāņu
- Physicochemical parameters
V1 — tilpums, kas izmantots paraugu titrēšanai ar standarta perhlorskābes šķīdumu, mililitros (ml).
Vo - tilpums, kas izmantots titrēšanas tukšajā paraugā ar standarta perhlorskābes šķīdumu, mililitros (ml);
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Adrese: Qingpu iela 147, Šouanas pilsēta, Pudzjanas apgabals, Čendu pilsēta, Sičuaņas province, Ķīna
Tālrunis: 86-18880477902
Produkti
Neorganiskie mikroelementi
- Organiskās mikroelementu
- svahili
- Pielāgots pakalpojums
- Ātrās saites
Uzņēmuma profils
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Gudžaratu | Noklikšķiniet, lai veiktu pieprasījumu | © Autortiesības — 2010.–2025. g.: Visas tiesības aizsargātas. | Vietnes karte POPULĀRĀKIE MEKLĒJUMI Tālrunis |
| Tālrunis | 86-18880477902 | javiešu | E-pasts |
| 8618880477902 | Ķīniešu | Franču | |
| Bird | Ķīniešu | Franču | Vācu Spāņu |
| Aquatic animals | Japāņu | Korejiešu | Arābu Grieķu |
| Turku | Itāļu | ||
| Ruminant animal g/head day | January 0.75 | Indonēziešu afrikandu valoda Zviedru |
Poļu
- Basku
- Katalāņu
- Physicochemical parameters
Hindi
Lao
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Šona
Bulgāru
- Sebuāņu
- This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
- The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
- Horvātu
Holandiešu
| Application object | Urdu Vjetnamiešu | Content in full-value feed (mg/kg) | Efficacy |
| Gudžaratu | Haitiešu | Hausu | kinjarvandu Hmongu Ungāru |
| Piglets and fattening pigs | Igbo | javiešu | Kannadu Khmeru Kurdu |
| Kirgizstāna | Latīņu | ||
| Bird | 300～400 | 45～60 | Maķedoniešu Malajiešu Malajalu |
| Aquatic animals | 200～300 | 30～45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
Norvēģu
- puštu
- Appearance: brownish-yellow granules
- Physicochemical parameters
Serbu
Sesoto
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Šona
Sindhu
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
svahili
Tadžiku
Tamilu
Telugu
Taizemes
| Application object | Urdu Vjetnamiešu | Content in full-value feed (mg/kg) | Efficacy |
| Jidišs | Jorubu | Zulu | kinjarvandu Oriju Turkmēņu |
| uiguru | 250～400 | 37.5～60 | 1. Improving the immunity of piglets, reducing diarrhea and mortality; 2. Improving palatability, increasing feed intake, increasing growth rate and improving feed conversion; 3. Make the pig coat bright and improve the carcass quality and meat quality. |
| Bird | 300～400 | 45～60 | 1. Improve feather glossiness; 2. improve the laying rate, fertilization rate and hatching rate of breeding eggs, and strengthen the coloring ability of egg yolk; 3. Improve anti-stress ability and reduce mortality; 4. Improve feed conversion and increase growth rate. |
| Aquatic animals | January 300 | 45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
| Ruminant animal g/head day | 2.4 | 1. Improve milk yield, prevent mastitis and foof rot, and reduce somatic cell content in milk; 2. Promote growth, improve feed conversion and improve meat quality. |
4. Manganese Amino Acid Chelate Feed Grade
- Product Name: Manganese Amino Acid Chelate Feed Grade
- Appearance: brownish-yellow granules
- Physicochemical parameters
a) Mn: ≥ 10.0%
b) Total amino acids: ≥ 19.5%
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
n=0, 1,2,...indicates chelated manganese for dipeptides, tripeptides, and tetrapeptides
Characteristics of Manganese Amino Acid Chelate Feed Grade
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
The product can improve the growth rate, improve feed conversion and health status significantly; and improve the laying rate, hatching rate and healthy chick rate of breeding poultry obviously;
Manganese is necessary for bone growth and connective tissue maintenance. It is closely related to many enzymes; and participates in carbohydrate, fat and protein metabolism, reproduction and immune response.
Usage and Efficacy of Manganese Amino Acid Chelate Feed Grade
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Breeding pig | 200～300 | 30～45 | 1. Promote the normal development of sexual organs and improve sperm motility; 2. Improve the reproductive capacity of breeding pigs and reduce reproductive obstacles. |
| Piglets and fattening pigs | 100～250 | 15～37.5 | 1. It is beneficial to improve immune functions, and improve anti-stress ability and disease resistance; 2. Promote growth and improve feed conversion significantly; 3. Improve meat color and quality, and improve lean meat percentage. |
| Bird | 250～350 | 37.5～52.5 | 1. Improve anti-stress ability and reduce mortality; 2. Improve laying rate, fertilization rate and hatching rate of breeding eggs, improve eggshell quality and reduce shell breaking rate; 3. Promote bone growth and reduce the incidence of leg diseases. |
| Aquatic animals | 100～200 | 15～30 | 1. Promote growth and improve its anti-stress ability and disease resistance; 2. Improve sperm motility and hatching rate of fertilized eggs. |
| Ruminant animal g/head day | Cattle 1.25 | 1. Prevent fatty acid synthesis disorder and bone tissue damage; 2. Improve reproductive capacity, prevent abortion and postpartum paralysis of female animals, reduce the mortality of calves and lambs, and increase the newborn weight of young animals. | |
| Goat 0.25 |
Part 6 FAB of Small Peptide-mineral Chelates
| S/N | F: Functional attributes | A: Competitive differences | B: Benefits brought by competitive differences to users |
| 1.52 | Selectivity control of raw materials | Select pure plant enzymatic hydrolysis of small peptides | High biological safety, avoiding cannibalism |
| 2 | Directional digestion technology for double protein biological enzyme | High proportion of small molecular peptides | More "targets", which are not easy to saturation, with high biological activity and better stability |
| 3 | Advanced pressure spray & drying technology | Granular product, with uniform particle size, better fluidity, not easy to absorb moisture | Ensure easy to use, more uniform mixing in complete feed |
| Low water content (≤ 5%), which greatly reduces the influence caused by vitamins and enzyme preparations | Improve the stability of feed products | ||
| 4 | Advanced production control technology | Totally enclosed process, high degree of automatic control | Safe and stable quality |
| 5 | Advanced quality control technology | Establish and improve scientific and advanced analytical methods and control means for detecting factors affecting product quality, such as acid-soluble protein, molecular weight distribution, amino acids and chelating rate | Ensure quality, ensure efficiency and improve efficiency |
Part 7 Competitor Comparison
Standard VS Standard
Comparison of peptide distribution and chelation rate of products
| Sustar's products | Proportion of small peptides（180-500） | Zinpro's products | Proportion of small peptides（180-500） |
| AA-Cu | ≥74% | AVAILA-Cu | 78% |
| AA-Fe | ≥48% | AVAILA-Fe | 59% |
| AA-Mn | ≥33% | AVAILA-Mn | 53% |
| AA-Zn | ≥37% | AVAILA-Zn | 56% |
| Sustar's products | Chelation rate | Zinpro's products | Chelation rate |
| AA-Cu | 94.8% | AVAILA-Cu | 94.8% |
| AA-Fe | 95.3% | AVAILA-Fe | 93.5% |
| AA-Mn | 94.6% | AVAILA-Mn | 94.6% |
| AA-Zn | 97.7% | AVAILA-Zn | 90.6% |
The ratio of small peptides of Sustar is slightly lower than that of Zinpro, and the chelation rate of Sustar's products is slightly higher than that of Zinpro's products.
Comparison of the content of 17 amino acids in different products
| Name of amino acids | Sustar's Copper Amino Acid Chelate Feed Grade | Zinpro's AVAILA copper | Sustar's Ferrous Amino Acid C helate Feed Grade | Zinpro's AVAILA iron | Sustar's Manganese Amino Acid Chelate Feed Grade | Zinpro's AVAILA manganese | Sustar's Zinc Amino Acid Chelate Feed Grade | Zinpro's AVAILA zinc |
| aspartic acid (%) | 1.88 | 0.72 | 1.50 | 0.56 | 1.78 | 1.47 | 1.80 | 2.09 |
| glutamic acid (%) | 4.08 | 6.03 | 4.23 | 5.52 | 4.22 | 5.01 | 4.35 | 3.19 |
| Serine (%) | 0.86 | 0.41 | 1.08 | 0.19 | 1.05 | 0.91 | 1.03 | 2.81 |
| Histidine (%) | 0.56 | 0.00 | 0.68 | 0.13 | 0.64 | 0.42 | 0.61 | 0.00 |
| Glycine (%) | 1.96 | 4.07 | 1.34 | 2.49 | 1.21 | 0.55 | 1.32 | 2.69 |
| Threonine (%) | 0.81 | 0.00 | 1.16 | 0.00 | 0.88 | 0.59 | 1.24 | 1.11 |
| Arginine (%) | 1.05 | 0.78 | 1.05 | 0.29 | 1.43 | 0.54 | 1.20 | 1.89 |
| Alanine (%) | 2.85 | 1.52 | 2.33 | 0.93 | 2.40 | 1.74 | 2.42 | 1.68 |
| Tyrosinase (%) | 0.45 | 0.29 | 0.47 | 0.28 | 0.58 | 0.65 | 0.60 | 0.66 |
| Cystinol (%) | 0.00 | 0.00 | 0.09 | 0.00 | 0.11 | 0.00 | 0.09 | 0.00 |
| Valine (%) | 1.45 | 1.14 | 1.31 | 0.42 | 1.20 | 1.03 | 1.32 | 2.62 |
| Methionine (%) | 0.35 | 0.27 | 0.72 | 0.65 | 0.67 | 0.43 | January 0.75 | 0.44 |
| Phenylalanine (%) | 0.79 | 0.41 | 0.82 | 0.56 | 0.70 | 1.22 | 0.86 | 1.37 |
| Isoleucine (%) | 0.87 | 0.55 | 0.83 | 0.33 | 0.86 | 0.83 | 0.87 | 1.32 |
| Leucine (%) | 2.16 | 0.90 | 2.00 | 1.43 | 1.84 | 3.29 | 2.19 | 2.20 |
| Lysine (%) | 0.67 | 2.67 | 0.62 | 1.65 | 0.81 | 0.29 | 0.79 | 0.62 |
| Proline (%) | 2.43 | 1.65 | 1.98 | 0.73 | 1.88 | 1.81 | 2.43 | 2.78 |
| Total amino acids (%) | 23.2 | 21.4 | 22.2 | 16.1 | 22.3 | 20.8 | 23.9 | 27.5 |
Overall, the proportion of amino acids in Sustar's products is higher than that in Zinpro's products.
Part 8 Effects of use
Effects of different sources of trace minerals on the production performance and egg quality of laying hens in the late laying period
Production Process
- Targeted chelation technology
- Shear emulsification technology
- Pressure spray & drying technology
- Refrigeration & dehumidification technology
- Advanced environmental control technology
Appendix A: Methods for the Determination of relative molecular mass distribution of peptides
Adoption of standard: GB/T 22492-2008
1 Test Principle:
It was determined by high performance gel filtration chromatography. That is to say, using porous filler as stationary phase, based on the difference in the relative molecular mass size of the sample components for separation, detected at the peptide bond of the ultraviolet absorption wavelength of 220nm, using the dedicated data processing software for the determination of relative molecular mass distribution by gel filtration chromatography (i.e., the GPC software), the chromatograms and their data were processed, calculated to get the size of the relative molecular mass of the soybean peptide and the distribution range.
2. Reagents
The experimental water should meet the specification of secondary water in GB/T6682, the use of reagents, except for special provisions, are analytically pure.
2.1 Reagents include acetonitrile (chromatographically pure), trifluoroacetic acid (chromatographically pure),
2.2 Standard substances used in the calibration curve of relative molecular mass distribution: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Instrument and equipment
3.1 High Performance Liquid Chromatograph (HPLC): a chromatographic workstation or integrator with a UV detector and GPC data processing software.
3.2 Mobile phase vacuum filtration and degassing unit.
3.3 Electronic balance: graduated value 0.000 1g.
4 Operating steps
4.1 Chromatographic conditions and system adaptation experiments (reference conditions)
4.1.1 Chromatographic column: TSKgelG2000swxl300 mm×7.8 mm (inner diameter) or other gel columns of the same type with similar performance suitable for the determination of proteins and peptides.
4.1.2 Mobile phase: Acetonitrile + water + trifluoroacetic acid = 20 + 80 + 0.1.
4.1.3 Detection wavelength: 220 nm.
4.1.4 Flow rate: 0.5 mL/min.
4.1.5 Detection time: 30 min.
4.1.6 Sample injection volume: 20μL.
4.1.7 Column temperature: room temperature.
4.1.8 In order to make the chromatographic system meet the detection requirements, it was stipulated that under the above chromatographic conditions, the gel chromatographic column efficiency, i.e., the theoretical number of plates (N), was not less than 10000 calculated on the basis of the peaks of the tripeptide standard (Glycine-Glycine-Glycine).
4.2 Production of relative molecular mass standard curves
The above different relative molecular mass peptide standard solutions with a mass concentration of 1 mg / mL were prepared by mobile phase matching, mixed in a certain proportion, and then filtered through an organic phase membrane with the pore size of 0.2 μm~0.5 μm and injected into the sample, and then the chromatograms of the standards were obtained. Relative molecular mass calibration curves and their equations were obtained by plotting the logarithm of relative molecular mass against retention time or by linear regression.
4.3 Sample treatment
Accurately weigh 10mg of sample in a 10mL volumetric flask, add a little mobile phase, ultrasonic shaking for 10min, so that the sample is fully dissolved and mixed, diluted with mobile phase to the scale, and then filtered through an organic phase membrane with a pore size of 0.2μm~0.5μm, and the filtrate was analyzed according to the chromatographic conditions in A.4.1.
5. Calculation of relative molecular mass distribution
After analyzing the sample solution prepared in 4.3 under the chromatographic conditions of 4.1, the relative molecular mass of the sample and its distribution range can be obtained by substituting the chromatographic data of the sample into the calibration curve 4.2 with GPC data processing software. The distribution of the relative molecular masses of the different peptides can be calculated by the peak area normalization method, according to the formula: X=A/A total×100
In the formula: X - The mass fraction of a relative molecular mass peptide in the total peptide in the sample, %;
A - Peak area of a relative molecular mass peptide;
Total A - the sum of the peak areas of each relative molecular mass peptide, calculated to one decimal place.
6 Repeatability
The absolute difference between two independent determinations obtained under conditions of repeatability shall not exceed 15% of the arithmetic mean of the two determinations.
Appendix B: Methods for the Determination of Free Amino Acids
Adoption of standard: Q/320205 KAVN05-2016
1.2 Reagents and materials
Glacial acetic acid: analytically pure
Perchloric acid: 0.0500 mol/L
Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
The samples were dried at 80°C for 1 hour.
Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask.
Quickly proceed to the next step to avoid the sample from absorbing ambient moisture
Add 25 mL of glacial acetic acid and mix well for no more than 5 min.
Add 2 drops of crystal violet indicator
Titrate with 0.0500 mol / L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to the end point.
Record the volume of standard solution consumed.
Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%) and is calculated according to the formula: X = C × (V1-V0) × 0.1445/M × 100%, in tne formula:
C - Concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445: Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
Appendix C: Methods for the Determination of Sustar's chelation rate
Adoption of standards: Q/70920556 71-2024
1. Determination principle (Fe as an example)
Amino acid iron complexes have very low solubility in anhydrous ethanol and free metal ions are soluble in anhydrous ethanol, the difference in solubility between the two in anhydrous ethanol was utilized to determine the chelation rate of amino acid iron complexes.
2. Reagents & Solutions
Anhydrous ethanol; the rest is the same as clause 4.5.2 in GB/T 27983-2011.
3. Steps of analysis
Do two trials in parallel. Weigh 0.1g of the sample dried at 103±2℃ for 1 hour, accurate to 0.0001g, add 100mL of anhydrous ethanol to dissolve, filter, filter residue washed with 100mL of anhydrous ethanol for at least three times, then transfer the residue into a 250mL conical flask, add 10mL of sulfuric acid solution according to clause 4.5.3 in GB/T27983-2011, and then perform the following steps according to clause 4.5.3 “Heat to dissolve and then let cool” in GB/T27983-2011. Carry out the blank test at the same time.
4. Determination of total iron content
4.1 The principle of determination is the same as clause 4.4.1 in GB/T 21996-2008.
4.2. Reagents & Solutions
4.2.1 Mixed acid: Add 150mL of sulfuric acid and 150mL of phosphoric acid to 700mL of water and mix well.
4.2.2 Sodium diphenylamine sulfonate indicator solution: 5g/L, prepared according to GB/T603.
4.2.3 Cerium sulfate standard titration solution: concentration c [Ce (SO4) 2] = 0.1 mol/L, prepared according to GB/T601.
4.3 Steps of analysis
Do two trials in parallel. Weigh 0.1g of sample, accurate to 020001g, place in a 250mL conical flask, add 10mL of mixed acid, after dissolution, add 30ml of water and 4 drops of sodium dianiline sulfonate indicator solution, and then perform the following steps according to clause 4.4.2 in GB/T21996-2008. Carry out the blank test at the same time.
4.4 Representation of results
The total iron content X1 of the amino acid iron complexes in terms of mass fraction of iron, the value expressed in %, was calculated according to formula (1):
X1=(V-V0)×C×M×10-3×100
In the formula: V - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V0 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L
5. Calculation of iron content in chelates
The iron content X2 in the chelate in terms of the mass fraction of iron, the value expressed in %, was calculated according to the formula: x2 = ((V1-V2) × C × 0.05585)/m1 × 100
In the formula: V1 - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V2 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L;
0.05585 - mass of ferrous iron expressed in grams equivalent to 1.00 mL of cerium sulfate standard solution C[Ce(SO4)2.4H20] = 1.000 mol/L.
m1-Mass of the sample, g. Take the arithmetic mean of the parallel determination results as the determination results, and the absolute difference of the parallel determination results is not more than 0.3%.
6. Calculation of chelation rate
Chelation rate X3, the value expressed in %, X3 = X2/X1 × 100
Appendix C: Methods for the Determination of Zinpro's chelation rate
Adoption of standard: Q/320205 KAVNO7-2016
1. Reagents and materials
a) Glacial acetic acid: analytically pure; b) Perchloric acid: 0.0500mol/L; c) Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
2.1 The samples were dried at 80°C for 1 hour.
2.2 Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
2.3 Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask
2.4 Quickly proceed to the next step to avoid the sample from absorbing ambient moisture.
2.5 Add 25mL of glacial acetic acid and mix well for no more than 5min.
2.6 Add 2 drops of crystal violet indicator.
2.7 Titrate with 0.0500mol/L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to green for 15s without changing color as the end point.
2.8 Record the volume of standard solution consumed.
2.9 Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%), calculated according to formula (1): X=C×(V1-V0) ×0.1445/M×100%...... .......(1)
In the formula: C - concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445 - Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
4. Calculation of chelation rate
The chelation rate of the sample is expressed as mass fraction (%), calculated according to formula (2): chelation rate = (total amino acid content - free amino acid content)/total amino acid content×100%.
Post time: Sep-17-2025
